The use of DNA microarrays for the detection of viruses in respiratory tract infections is a promising technique, although it must be purified, cheapened and simplified before its introduction into clinical practice" />

December 2008. Volume 4. Number 4

The use of DNA microarrays for the detection of viruses in respiratory tract infections is a promising technique, although it must be purified, cheapened and simplified before its introduction into clinical practice

 
 
 
 
 
 
 
 
 
 
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AVC | Critically appraised articles

Chiu CY, Urisman A, Greenhow TL, Rouskin S, Yagi S, Schnurr D, et al. Utility of DNA microarrays for detection of viruses in acute respiratory tract infections in children. J Pediatr. 2008;153:76-83
Reviewers: Andrés de Llano JM1, Ochoa Sangrador C2.
1Servicio de Pediatría. Hospital General del Río Carrión. Palencia. España.
2Servicio de Pediatría. Hospital Virgen de la Concha. Zamora. España.
Correspondence: Jesús María Andrés de Llano. Email: jmandres@ono.com
Reception date: 21/10/2008
Acceptance date: 23/10/2008
Publication date: 01/12/2008

Abstract

Authors' conclusions: considering its favorable profile with regard to sensibility, specificity and wide spectrum, the viral test based on microarrays promises to be an important diagnostic help for the clinical pediatrician in the detection of infections of the respiratory tract.

Reviewers' comantary: in spite of the advantages of these new technologies, they do not currently seem to be applicable in the welfare practice, in view of his high cost and complexity.
Lowering the price, simplification and automatization are necessary before they replace the technique nowadays in use. Likewise the time of delay for obtaining result will have to be shorter and the consistency, reproducibility and clinical profitability must be proved in new studies.

How to cite this article

Andrés de Llano J, Ochoa Sangrador C. El uso de micromatrices de DNA para la detección de virus en infecciones del tracto respiratorio es una técnica prometedora, aunque debe depurarse, abaratarse y simplificarse antes de su introducción en la práctica clínica. Evid Pediatr. 2008;4:75.

AVC | Critically appraised articles

Chiu CY, Urisman A, Greenhow TL, Rouskin S, Yagi S, Schnurr D, et al. Utility of DNA microarrays for detection of viruses in acute respiratory tract infections in children. J Pediatr. 2008;153:76-83
Reviewers: Andrés de Llano JM1, Ochoa Sangrador C2.
1Servicio de Pediatría. Hospital General del Río Carrión. Palencia. España.
2Servicio de Pediatría. Hospital Virgen de la Concha. Zamora. España.
Correspondence: Jesús María Andrés de Llano. Email: jmandres@ono.com
Reception date: 21/10/2008
Acceptance date: 23/10/2008
Publication date: 01/12/2008

How to cite this article

Andrés de Llano J, Ochoa Sangrador C. El uso de micromatrices de DNA para la detección de virus en infecciones del tracto respiratorio es una técnica prometedora, aunque debe depurarse, abaratarse y simplificarse antes de su introducción en la práctica clínica. Evid Pediatr. 2008;4:75.

References

  1. Genoma España. Microarrays y Biochips de ADN. Informe de Vigilancia Tecnológica. Madrid: Fundación General de la Universidad Autónoma de Madrid; 2002. Fecha de consulta: 1 de octubre de 2008. Disponible en: http://www.gen-es.org/12_publ/docs/Microarrays.pdf
  2. Syrmis MW, Whiley DM, Thomas M, Mackay IM, Williamson J, Siebert DJ, et al. A sensitive, specific, and cost-effective multiplex reverse transcriptase-PCR assay for the detection of seven common respiratory viruses in respiratory samples. J Mol Diagn. 2004;6:125-31.
  3. Noyola DE, Demmler GJ. Effect of rapid diagnosis on management of influenza A infections. Pediatr Infect Dis J. 2000;19:303-7.
01/12/2008

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